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influenzae serotype c  (ATCC)
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ATCC influenzae serotype c
Primers sequences used in this study.
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Primers sequences used in this study.

Journal: The Brazilian Journal of Infectious Diseases

Article Title: Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae , and Streptococcus pneumoniae in clinical samples

doi: 10.1016/j.bjid.2016.04.005

Figure Lengend Snippet: Primers sequences used in this study.

Article Snippet: Twenty nine reference strains were used as positive controls as follows: N. meningitidis serogroup A (ATCC 13077), N. meningitidis serogroup B (ATCC 13090), N. meningitidis serogroup C (ATCC 13102), N. meningitidis serogroup W135 (ATCC 35559), H. influenzae (ATCC 33391), H. influenzae aegyptius (ATCC 11116), H. influenzae NT (ATCC 49247), H. influenzae serotype a (ATCC 9006), H. influenzae serotype b (ATCC 33533) H. influenzae serotype c (ATCC 9007), H. influenzae serotype d (ATCC 9008), H. influenzae serotype e (ATCC 8142), H. influenzae serotype f (ATCC 9833), S. pneumoniae (ATCC 33400), S. pneumoniae serotype 14 (ATCC 6314), S. pneumoniae serotype 3 (ATCC 6303), S. pneumoniae serotype 33 (ATCC 8333), S. pneumoniae serotype 41 (ATCC 10341), S. pneumoniae serotype 51 (ATCC 10351), S. pneumoniae serotype 19F (ATCC 49619), S. pneumoniae serotype 61 (ATCC 10361), S. pneumoniae serotype 19A (ATCC 700673), S. pneumoniae serotype 14 (ATCC 700672), S. pneumoniae serotype 9V (ATCC 700671), S. pneumoniae serotype 6B (ATCC 700670), S. pneumoniae serotype 4 (ATCC BAA-334), S. pneumoniae serotype 6A (ATCC BAA-659), S. pneumoniae serotype 5 (ATCC BAA-341).

Techniques: Amplification, Multiplex Assay

Sensitivity of in-house-PCR and commercial PCR-based detection kit.

Journal: The Brazilian Journal of Infectious Diseases

Article Title: Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae , and Streptococcus pneumoniae in clinical samples

doi: 10.1016/j.bjid.2016.04.005

Figure Lengend Snippet: Sensitivity of in-house-PCR and commercial PCR-based detection kit.

Article Snippet: Twenty nine reference strains were used as positive controls as follows: N. meningitidis serogroup A (ATCC 13077), N. meningitidis serogroup B (ATCC 13090), N. meningitidis serogroup C (ATCC 13102), N. meningitidis serogroup W135 (ATCC 35559), H. influenzae (ATCC 33391), H. influenzae aegyptius (ATCC 11116), H. influenzae NT (ATCC 49247), H. influenzae serotype a (ATCC 9006), H. influenzae serotype b (ATCC 33533) H. influenzae serotype c (ATCC 9007), H. influenzae serotype d (ATCC 9008), H. influenzae serotype e (ATCC 8142), H. influenzae serotype f (ATCC 9833), S. pneumoniae (ATCC 33400), S. pneumoniae serotype 14 (ATCC 6314), S. pneumoniae serotype 3 (ATCC 6303), S. pneumoniae serotype 33 (ATCC 8333), S. pneumoniae serotype 41 (ATCC 10341), S. pneumoniae serotype 51 (ATCC 10351), S. pneumoniae serotype 19F (ATCC 49619), S. pneumoniae serotype 61 (ATCC 10361), S. pneumoniae serotype 19A (ATCC 700673), S. pneumoniae serotype 14 (ATCC 700672), S. pneumoniae serotype 9V (ATCC 700671), S. pneumoniae serotype 6B (ATCC 700670), S. pneumoniae serotype 4 (ATCC BAA-334), S. pneumoniae serotype 6A (ATCC BAA-659), S. pneumoniae serotype 5 (ATCC BAA-341).

Techniques:

Agarose gel of a multiplex PCR showing the detection of the three pathogens from three different samples. Lane 1: 100 bp DNA ladder; lane 2: Neisseria meningitidis ( nspA gene with 481 bp); lane 3: Haemophilus influenzae (P6 gene with 273 bp); lane 4: Streptococcus pneumoniae ( ply gene with 1000 bp); lane 5: negative control.

Journal: The Brazilian Journal of Infectious Diseases

Article Title: Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae , and Streptococcus pneumoniae in clinical samples

doi: 10.1016/j.bjid.2016.04.005

Figure Lengend Snippet: Agarose gel of a multiplex PCR showing the detection of the three pathogens from three different samples. Lane 1: 100 bp DNA ladder; lane 2: Neisseria meningitidis ( nspA gene with 481 bp); lane 3: Haemophilus influenzae (P6 gene with 273 bp); lane 4: Streptococcus pneumoniae ( ply gene with 1000 bp); lane 5: negative control.

Article Snippet: Twenty nine reference strains were used as positive controls as follows: N. meningitidis serogroup A (ATCC 13077), N. meningitidis serogroup B (ATCC 13090), N. meningitidis serogroup C (ATCC 13102), N. meningitidis serogroup W135 (ATCC 35559), H. influenzae (ATCC 33391), H. influenzae aegyptius (ATCC 11116), H. influenzae NT (ATCC 49247), H. influenzae serotype a (ATCC 9006), H. influenzae serotype b (ATCC 33533) H. influenzae serotype c (ATCC 9007), H. influenzae serotype d (ATCC 9008), H. influenzae serotype e (ATCC 8142), H. influenzae serotype f (ATCC 9833), S. pneumoniae (ATCC 33400), S. pneumoniae serotype 14 (ATCC 6314), S. pneumoniae serotype 3 (ATCC 6303), S. pneumoniae serotype 33 (ATCC 8333), S. pneumoniae serotype 41 (ATCC 10341), S. pneumoniae serotype 51 (ATCC 10351), S. pneumoniae serotype 19F (ATCC 49619), S. pneumoniae serotype 61 (ATCC 10361), S. pneumoniae serotype 19A (ATCC 700673), S. pneumoniae serotype 14 (ATCC 700672), S. pneumoniae serotype 9V (ATCC 700671), S. pneumoniae serotype 6B (ATCC 700670), S. pneumoniae serotype 4 (ATCC BAA-334), S. pneumoniae serotype 6A (ATCC BAA-659), S. pneumoniae serotype 5 (ATCC BAA-341).

Techniques: Agarose Gel Electrophoresis, Multiplex Assay, Negative Control

Agarose gel showing the differentiation of capsulated and non-capsulated Haemophilus influenzae strains. Lane 1: 100 bp DNA ladder; lane 2: capsulated H. influenzae ( bexA and P6 genes with 343 bp and 273 bp respectively); lane 3: non-capsulated H. influenzae (HiNT), ( bexA gene with 343 bp); lane 4 negative control.

Journal: The Brazilian Journal of Infectious Diseases

Article Title: Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae , and Streptococcus pneumoniae in clinical samples

doi: 10.1016/j.bjid.2016.04.005

Figure Lengend Snippet: Agarose gel showing the differentiation of capsulated and non-capsulated Haemophilus influenzae strains. Lane 1: 100 bp DNA ladder; lane 2: capsulated H. influenzae ( bexA and P6 genes with 343 bp and 273 bp respectively); lane 3: non-capsulated H. influenzae (HiNT), ( bexA gene with 343 bp); lane 4 negative control.

Article Snippet: Twenty nine reference strains were used as positive controls as follows: N. meningitidis serogroup A (ATCC 13077), N. meningitidis serogroup B (ATCC 13090), N. meningitidis serogroup C (ATCC 13102), N. meningitidis serogroup W135 (ATCC 35559), H. influenzae (ATCC 33391), H. influenzae aegyptius (ATCC 11116), H. influenzae NT (ATCC 49247), H. influenzae serotype a (ATCC 9006), H. influenzae serotype b (ATCC 33533) H. influenzae serotype c (ATCC 9007), H. influenzae serotype d (ATCC 9008), H. influenzae serotype e (ATCC 8142), H. influenzae serotype f (ATCC 9833), S. pneumoniae (ATCC 33400), S. pneumoniae serotype 14 (ATCC 6314), S. pneumoniae serotype 3 (ATCC 6303), S. pneumoniae serotype 33 (ATCC 8333), S. pneumoniae serotype 41 (ATCC 10341), S. pneumoniae serotype 51 (ATCC 10351), S. pneumoniae serotype 19F (ATCC 49619), S. pneumoniae serotype 61 (ATCC 10361), S. pneumoniae serotype 19A (ATCC 700673), S. pneumoniae serotype 14 (ATCC 700672), S. pneumoniae serotype 9V (ATCC 700671), S. pneumoniae serotype 6B (ATCC 700670), S. pneumoniae serotype 4 (ATCC BAA-334), S. pneumoniae serotype 6A (ATCC BAA-659), S. pneumoniae serotype 5 (ATCC BAA-341).

Techniques: Agarose Gel Electrophoresis, Negative Control